Initial Stage of Formation of Spontaneous Invasive Populations of Garden Lupine (Lupinus polyphyllus Lindl.) at the Northern Limit of Its Secondary Distribution Range in the Veps Forest Nature Park

A weed plant of the species Lupinus polyphyllus was found in settlements located on territory natural park “Vepssky forest” Leningrad region. The is known as a transforming southern regions Russia. distribution L. within Vepsian forest occurs not due to migration north along transport routes, one might assume, but flight from gardens and formation spontaneous invasive populations. goal study identify molecular biochemical characteristics garden lupine at northern borders its secondary range. To interpret obtained data, task also included comparison intraspecific variability between “old” populations (in Finland Central Russia, studied by us earlier) “new” naturalizing population forest. search for localities Vepssky Les carried out route method with geobotanical descriptions experimental sites (5 m × 5 m). DNA isolated eight herbarium specimens (MHA, LE) fifteen collected Les”. reveal internal structure phylogenetic relationships populations, networks nuclear chloroplast haplotypes cluster analysis (UPGMA) SplitsTree program were used. total content polyphenols flavonoids leaves determined spectrophotometrically. low inter-locality ITS indicates that Russia North (St. Petersburg Vepsskaya Pushcha) have same origin. Analysis intergenic spacer (rpl32–trnL) intrapopulation diversity suggests presence microevolutionary processes near limits range polyphyllus. high reveals adaptive capabilities area. Evidence neglected may soon become invasive, has already happened other regions.